TABLE 2.
Kinetic parameters of PDEs
The kinetics data of LmjPDEB1 and human PDEs are cited from Refs. 28, 43, 45, 52, and 53. ND, not determined.
| cAMP |
cGMP |
|||||
|---|---|---|---|---|---|---|
| Km | kcat | kcat/Km | Km | kcat | kcat/Km | |
| μm | s−1 | s−1μm−1 | μm | s−1 | s−1μm−1 | |
| TcrPDEC (270–614) | 21.3 ± 2.1 | 30.7 ± 0.9 | 1.4 ± 0.1 | 87.0 ± 6.2 | 16.7 ± 0.4 | 0.19 ± 0.02 |
| LmjPDEB1 (582–940) | 20.6 ± 3.4 | 2.7 ± 0.3 | 0.13 ± 0.01 | >1000 | ND | ND |
| PDE4D2 | 1.5 ± 0.2 | 3.9 ± 0.3 | 2.7 ± 0.4 | 990 ± 100 | 5.2 ± 0.8 | 0.053 ± 0.008 |
| PDE5A1 (535–860) | ND | ND | ND | 5.1 ± 1.3 | 1.3 ± 0.3 | 0.27 ± 0.08 |
| PDE7A1 (130–482) | 0.20 ± 0.03 | 1.6 ± 0.2 | 7.9 ± 0.9 | 3900 ± 700 | 6.8 ± 1.3 | 0.0018 ± 0.0001 |
| PDE8A1 (480–820) | 1.8 ± 0.1 | 4.0 ± 0.1 | 2.2 ± 0.1 | 1600 ± 100 | 1.6 ± 0.2 | 0.001 ± 0.0001 |
| PDE10A2 (449–789) | 0.056 ± 0.005 | 0.33 ± 0.02 | 5.9 ± 0.6 | 4.4 ± 0.3 | 1.2 ± 0.1 | 0.27 ± 0.01 |