FIGURE 4.

Kinetics of DNA heterology bypass by RAD54, BLM, and RecQ1. A, experimental design. RAD51-generated 3′-JMs with 2-, 4-, 8-, 16-, 24-, 48-, and 96-bp heterologies (denoted by the black box) were incubated with RAD54, BLM, or RECQ1. The asterisks indicate ³²P-label. nt, nucleotide. B–D, the kinetics of branch migration by RAD54 (200 nm), BLM (20 nm), or RECQ1 (200 nm) on the deproteinized and purified JMs (0.9 nm) containing heterologies of the indicated length. The data are shown in supplemental Fig. S3. The error bars indicate S.E. no mis, no mismatches.