FIGURE 7.

Effect of human RPA on heterology bypass promoted by BLM and RAD54. A–D, various amounts of human RPA were added to the 3′-JMs (0.9 nm) containing heterologous DNA regions of the indicated lengths, prior to the addition of RAD54 (200 nm) (A and C) or BLM (20 nm) (B and D). The RAD54-promoted BM with JMs containing 24- and 96-bp heterology regions were carried out for 10 and 20 min, respectively, at 30 °C; the BLM-promoted BM was carried out for 1 h at 37 °C. E and F, the data from panels A–D are shown as graphs. The error bars indicate S.E.