TABLE 1.

Optimal conditions for four-strand branch migration on plasmid-length 3′-JMs

See the reaction conditions under “Experimental Procedures.”

Protein	RAD54	BLM	RECQ1
Optimal Mg2+ acetate concentration in the presence of 2 mm ATP and ATP regeneration system	3 mm	1 mm	0.5–2 mm
Optimal protein monomer/α-structure binding stoichiometry	150	20	120
Optimal temperature	30 °C	37 °C	37 °C
Time to complete branch migration of 1 nm (molecules) α-structures	10 min	90 min	240 min