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. 2012 Feb 17;287(15):11968–11980. doi: 10.1074/jbc.M111.336834

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Myonectin is secreted as a multimeric protein that can form heteromeric complexes with other CTRP family members. A, immunoblot analysis of cell pellet (P) or supernatant (S) from transfected HEK 293T cells expressing pCDNA3.1 control vector or FLAG epitope-tagged myonectin. B and C, immunoblot analysis of myonectin subjected to N-glycosidase F (PNGase F) (B) or β-mercaptoethanol (β-ME) (C) treatment. D, native gel immunoblot analysis of myonectin. Arrows in panels A and B, indicate possible isoforms of myonectin resulting from differential glycosylation. Arrows in panel D indicate different oligomeric forms of myonectin. E, immunoprecipitation (IP) followed by immunoblot (IB) analysis of supernatants from HEK 293T cells expressing a combination of FLAG-tagged myonectin and HA-tagged adiponectin or CTRPs.