Myonectin is produced by skeletal muscle and circulates in plasma.
A, immunoblot analysis of supernatant from HEK 293T cells expressing control vector or FLAG-tagged myonectin using a rabbit anti-myonectin antibody. B, immunoblot detection of myonectin in mouse skeletal muscle lysate. Arrows indicate possible isoforms of myonectin resulting from differential glycosylation. C, immunoblot detection of myonectin in mouse serum. D, estimation of serum concentration of myonectin in wild-type 12-week-old C57BL/6J male mice. Purified recombinant myonectin was used to construct a standard curve. AU, arbitrary units.