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High-fat diet reduces myonectin expression and its circulating levels. A, immunoblot quantification of serum myonectin levels in mice fed a high-fat diet (HFD) or an isocaloric matched low-fat diet (LFD) for 12 weeks. B, quantitative PCR analysis of myonectin expression in calf muscle isolated from low-fat diet- or high-fat diet-fed male mice. All data are presented as mean ± S.E. relative to low-fat diet-fed mice (n = 8 mice/group; **, p < 0.01).
