FIGURE 8.

Recombinant myonectin administration reduces serum nonesterified free fatty acid levels in mice. A, immunoblot detection of FLAG epitope-tagged myonectin in mouse serum before and after recombinant protein injection. B, immunoblot quantification revealed an ∼60% elevation in serum myonectin levels above normal baseline levels after recombinant protein injection (n = 5 per group). C and D, male mice were injected intraperitoneally with vehicle or myonectin (5 μg/g of body weight), and sera were harvested every hour for 5 h following recombinant protein administration. Food was removed 2 h prior to protein injection. Serum nonesterified fatty acid (C) and triglyceride (D) levels were quantified (n = 5 mice/group). All data are presented as mean ± S.E. (*, p < 0.05).