Recombinant myonectin has no effect on adipocytes or adipose tissue lipolysis.
A, NEFA concentration in media of 3T3-L1 adipocytes treated for 1 h with vehicle, isoproterenol (1 μm), myonectin (5 μg/ml), or a combination of myonectin and isoproterenol (Iso, n = 12 per group). N.S., not significant. All data are presented as mean ± S.E. relative to vehicle control (***, p < 0.005). B, time course of NEFA release into conditioned media of adipose tissue (epididymal fat pad) explants treated with vehicle, isoproterenol (1 μm), myonectin (5 μg/ml), or a combination of myonectin and isoproterenol (n = 6 per group).