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. 2012 Feb 13;287(15):12006–12015. doi: 10.1074/jbc.M111.280701

FIGURE 3.

FIGURE 3.

Effect of miRNA modulation on promoter activity of AQP4 M1. A, the luciferase reporter construct containing the entire 2.1-kb promoter was transfected into astrocytoma cells with the anti- or pre-miRNAs. Luciferase luminescence readings were obtained 48 h post-transfection. Relative luminescence was obtained by normalizing the value against respective negative control plasmids. Statistical analyses were done using t tests. *, p < 0.05; **, p < 0.01 compared with control. Error bars represent the mean ± S.E. (n = 3). B, AQP4 immunoreactivities in astrocytoma cells transfected with anti- or pre-miRNAs. Human astrocytoma cells were co-transfected with promoter/AQP4 CDS constructs together with anti- or pre-miR-130a, -152, -668, -939, or -1280. Control (Ctrl) cells were transfected with AQP4 CDS constructs and scrambled miRNAs. The cells were fixed, immunolabeled with anti-AQP4 antibodies (green), and stained with the nuclear stain DAPI (blue).