FIGURE 4.

miR-1280/-130a mutant constructs. Using the miR-668 deletion construct (which contains recognition sites for miR-1280 and miR-130a), three other plasmids were generated such that they contained miR-1280 and/or miR-130a recognition site mutations. Mutated sites are represented by xxxxx. The respective mutated constructs were transfected into HeLa cells, and the relative promoter activity of the constructs were obtained with respect to the miR-1280/-130a double mutant construct. The predicted recognition site of miR-939 was not taken into consideration as these constructs were transfected into HeLa cells, which do not express miR-939. Statistical analyses were done using t tests. *, p < 0.05; **, p < 0.01 compared with control. Error bars represent the mean ± S.E. (n = 3).