FIGURE 8.

Expression levels of AQP4 and miR-130a in ischemic rat injected with LNA anti miR-130a. A, changes in AQP4 and miR-130a expression levels in the brain samples were quantitated using quantitative PCR and stem-loop qRT-PCR, respectively. Data shown are the mean ± S.D. B, infarct volume of rat treated with LNA anti miR-130a. Error bars represent the mean ± S.E. (n = 3). AQP4 protein expression correlated with mRNA levels (see inset). C, histological analysis of brain sections. 2,3,5-Triphenyltetrazolium chloride-stained coronal brain sections (2 mm thick) of rats injected with 50 pmol of LNA anti miR-130a are shown. Intracerebroventricular injections were given immediately after the removal of the suture. Surviving cells stained red, whereas dead cells remained white. MCAo, middle cerebral artery occlusion.