TABLE 2.
Stem-loop PCR for miRNA expression levels
Cycle threshold (CT) values of selected miRNAs in astrocytoma and HeLa cells. CT values for respective miRNAs were determined from 10 ng of total RNA reverse transcribed using miRNA-specific stem-loop primers. NTC refers to non-template controls. 18 S expression was used as positive control. Data shown are the mean ± S.D. (n = 3).
| miRNA |
CT values |
|
|---|---|---|
| Astrocytoma | HeLa | |
| miR-130a | 28.22 ± 0.217 | 28.37 ± 0.109 |
| miR-152 | 30.82 ± 0.049 | Undetermined |
| miR-668 | 32.26 ± 0.107 | Undetermined |
| miR-939 | 30.75 ± 0.095 | Undetermined |
| miR-1280 | 19.19 ± 0.201 | 30.12 ± 0.126 |
| NTC | Undetermined | Undetermined |
| 18 S | 9.23 ± 0.188 | 9.51 ± 0.216 |