FIGURE 4.

β-Catenin accumulation in LRP5 and LRP6 KD preadipocytes. Confluent 3T3-L1 preadipocytes were treated with either 100 nm insulin or 75 ng/ml Wnt3a for 3 or 6 h, and total protein was extracted for Western blot analysis. Band intensities for β-catenin levels were determined using Image J software, and fold changes were calculated as compared with untreated (ut) cells. A, Wnt3a stimulation. The error bars show S.E. (n = 4). * indicates a p value < 0.05, and ¤ indicates a p value < 0.06. B, insulin stimulation. The error bars show S.E. (n = 5). Con, control; ut, untreated; Beta-cat, β-catenin.