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LRP5 and insulin/IGF-1 receptor immunoprecipitation studies. A, LRP5 was immunoprecipitated from 3T3-L1 preadipocytes after insulin (100 nm) or Wnt3a (75 ng/ml) stimulations at various time points and blotted with anti-LRP5 and anti-IR/IGF1R antibodies. B, liver, white adipose, and brown adipose tissue samples from WT and LRP5 KO mice were immunoprecipitated with anti-LRP5 antibody and blotted with anti-LRP5 and anti-IR antibody. n = 4 for KO and n = 3 for WT. ut, untreated.
