Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Feb 15;287(15):12111–12120. doi: 10.1074/jbc.M111.327023

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 5. — Knockdown of corepressors accelerates free HDAC3 degradation. A, pulse-chase analysis using the N-19 antibody to detect free HDAC3 in control and corepressor-depleted 293T cells. B, kinetic modeling of pulse-chase reactions of free HDAC3 under conditions of variable corepressor concentration ([CoR] = 1 or 0.1) and variable degradation rate constant of free HDAC3 (k_d = 10 or 20). k_a, association rate constant of HDAC3 and corepressors. The results confirmed that reduced expression of corepressors will slow the process of free HDAC3 turnover unless the degradation rate has been increased concurrently. C, pulse-chase analysis as performed in A in control and corepressor-depleted HeLa (left panel) and SW480 (right panel) cells.