FIGURE 5.

Down-regulation of the expression of CXCL12 and CXCR4 by inhibiting mTOR pathway in MKN-45 cells. A, compounds targeting the PI3K/mTOR pathway down-regulated CXCR4 protein expression. MKN-45 cells were treated with PI-103, rapamycin, or PP-242 for 6 h, and CXCR4 protein was analyzed by immunoblotting. GAPDH was employed as a loading control. Data shown are representative of at least three independent experiments. B, PI3K pathway inhibitors inhibited the secretion of CXCL12. The supernatant was collected from the culture of MKN-45 cells after treatment with inhibitors for 24 h, and the CXCL12 level was assessed by human CXCL12 ELISA kit. The data (mean ± S.D. of three independent experiments) shown are fold change relative to the CXCL12 level in control group. *, p < 0.05; **, p < 0.01, difference with control group.