FIGURE 2.

Localization of Alix and Ozz in primary myotubes. A, representative confocal microscopy images showing Alix and Ozz localization in proliferating (D0) and differentiated (D3) primary myoblasts. In myotubes, Ozz and Alix displayed a punctated distribution pattern and were colocalized in discrete puncta (arrowheads) and in the proximity of the nucleus. Scale bar, 10 μm. B, computational analyses of several confocal images from Ozz wild-type or knock-out D3 myotubes (n = 5–6) confirmed the colocalization of the two fluorescent signals. The right panel shows the Pearson's correlations. The colocalization between Alix (green fluorescence) and Ozz (red fluorescence) is also shown as scatter plots (cytofluorograms) in the center and left panels. Ozz^−/− cells were included in this analysis as negative control. Standard deviations (error bars) and p values (***, p < 0.005) confirmed that the analysis was statistically significant.