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Inhibition of MMPs and laminin signaling regulate neuronal survival in vitro. Upper panel, MAP2 immunostaining of control (cnt), FN439-treated, laminin antibody (α-LM)-treated cultures, and cultures treated with both FN-439 and α-LM. Lower panel, quantification of neuron numbers (n = 10; means ± S.E.) Asterisks, p < 0.05 (one-way ANOVA). Neurons were incubated from DIV5 to DIV7. 50 μm FN439, 50 μg/ml α-LM, and 50 μg/ml anti-integrin β1 antibody (α-β1) were used.
