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. 2012 Feb 20;287(15):12184–12194. doi: 10.1074/jbc.M111.297671

FIGURE 6.

FIGURE 6.

In vivo injection of FN-439 inhibits MMP activity and suppresses apoptosis. A, in situ zymography. FN-439 was injected at P2, and its effect was analyzed at P4. Left panel, CA3 region of control and FN-439-injected animal. DAPI staining (left) and gelatin-FITC (right). Right panel, fluorescence intensities of gelatin-FITC. CA3 regions of the control and FN-439-injected animals were compared (n = 5; means ± S.E.). Asterisk, p < 0.01 (Student's t test). B, immunostaining of laminin. Left panel, CA3 regions of the control and FN-439-treated animals are shown. Resolution of fluorescent intensities is 0–254 levels, with the color code for intensity shown as a scale bar. Right panel, intensity of laminin immunostaining signals (n = 6; means ± S.E.). Asterisk, p < 0.0001 (Student's t test). C, c-cas3+ neurons in the control, FN-439-injected and anti-integrin β1 antibody (α-β1)-injected animals were analyzed for CA1, CA3 pyramidal cells, and CA1 SO interneurons (n = 10; means ± S.E.). Asterisks, p < 0.05 (one-way ANOVA). cnt, control.