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. 2012 Feb 27;287(15):12195–12203. doi: 10.1074/jbc.M111.315804

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Histone H3 is modified with O-GlcNAc. Biochemical validation of O-GlcNAc on histone H3. A, H3 was immunoprecipitated, and subsequently blotted for O-GlcNAc using the CTD 110.6 antibody. B, reciprocal IP was performed using the O-GlcNAc antibody RL2, and blotted for H3. C, O-GlcNAc was also detected on histones immunoprecipitated from UCD melanoma cells, in addition to an increased signal for O-GlcNAc after incubation with PUGNAc. D, 1 m GlcNAc competition during primary antibody incubation was used to validate the specificity of the CTD 110.6 antibody in HeLa cells.