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. 2012 Feb 27;287(15):12195–12203. doi: 10.1074/jbc.M111.315804

FIGURE 4.

FIGURE 4.

O-GlcNAc inhibits mitosis-specific phosphorylations on H3. O-GlcNAc inhibits several histone H3 phosphorylation sites. Asynchronous cells incubated with PUGNAc (A) or overexpressing HA-tagged OGT (B) displayed a weaker signal for H3S10ph when compared with the respective controls. Mitotic cells incubated with PUGNAc (C) or overexpressing HA-tagged OGT (D) displayed weaker signals for S10ph, S28ph, and T32ph as compared with their relative controls. *, p < 0.05, as calculated by paired two-sample t test.