FIGURE 5.

Noggin mediates adipocyte differentiation via Pax-1. A, cell lysates obtained from normal and obese MSC were detected for presence of Pax-1 by Western blot using anti-Pax-1 antibody. A representative blot is shown here. B, MSC with Pax-1 abrogation by stable transfection using Pax-1 shRNA (MSC^shPax-1) were subjected to adipocyte differentiation in the presence and absence of LY294002 and rapamycin. As a control, MSC stably transfected with a scramble construct (MSC^scrPax-1) was used. After 14 days, the presence of adipocytes was detected by Oil Red O staining. All the experiments were repeated three times (n = 3; *, p < 0.05).