FIGURE 4.

NF-κB induces ICAM-1 expression after Erg inhibition. A, Erg siRNA or control siRNA-treated HUVEC were transduced with AdIκBαSR or AdLacZ. ICAM-1 mRNA levels were measured by quantitative RT-PCR and results are expressed as fold-change compared with control siRNA AdLacZ-treated. n = 5. B, Erg or control Genebloc-transfected HUVEC were treated with BAY-11-7085 or dimethyl sulfoxide (DMSO) control. ICAM-1 protein levels were measured by Western blot using an anti-ICAM-1 antibody and normalized to levels of GAPDH (n = 3). *, p < 0.05; **, p < 0.01.