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. 2012 Feb 27;287(15):12343–12347. doi: 10.1074/jbc.C112.352161

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Effects of mutations in the N- and C-terminal DNA binding domains. A, mutants of the F1-C60 polypeptide. B, the F1-C60 polypeptide and mutants were tested for DNA binding, and the mean data ± S.D. from at least three independent experiments are shown. C, the F1-C60 polypeptide and mutants (0.5, 0.75, and 1 μm) were tested with RAD51 in the D-loop reaction. The results were quantified and plotted; error bars are mean ± S.D. from at least three independent experiments. D, mutants of the F3 fragment. E, the F3 fragment and mutants were tested for DNA binding, and the mean data ± S.D. from at least three independent experiments are shown. F, the F3 fragment and mutants (0.5, 0.75, and 1 μm) were tested with RAD51 in the D-loop reaction. The results were quantified and plotted; error bars are mean ± S.D. from at least three independent experiments.