FIGURE 1.

Formation and repair of Acr-induced DNA adducts in NHBE and NHLF. Exponentially growing cells were treated with different concentrations of Acr for 6 h at 37 °C. A and B, the genomic DNA was isolated, and the α-OH- and γ-OH-Acr-dG adducts were determined by ³²P-post labeling/two-dimensional TLC/HPLC method as described previously (14, 19). C, cells treated with Acr (100 μm for 6 h) were incubated in normal culture medium for different time (0–24 h), and the unrepaired α- and γ-Acr-dG adducts in the genomic DNA were quantified as in B. D, Acr-DNA adducts formed in the genomic DNA of Acr-treated cells were detected by the UvrABC incision method as described previously (14, 19).