FIGURE 3.

Effect of Acr treatment on NER and BER. A, NHBE, NHLF, and A549 (lung adenocarcinoma) were treated with different concentrations of Acr at 37 °C, cell extracts were prepared, and the in vitro DDR was carried out by the same method as described previously (14, 19, 27). Upper panels are DNA bands stained with ethidium bromide representing the input DNA, and lower panels are radioautograms of the same gel representing the extent of repair synthesis. B, control cell lysates were treated with different concentrations of Acr directly and DDR was carried out the same as in A. UV-irradiated (A, panel 1, and B, panel 3) and H₂O₂-modified (A, panel 2, and B, panel 4) pUC18 DNA was used as DNA substrates to detect NER and BER capacity, respectively. The relative repair efficiencies of cell lysates of different treatments were shown in the right. Note that this result shows that Acr treatment inhibits both NER and BER.