Skip to main content
. 2012 Jan 10;287(15):12395–12404. doi: 10.1074/jbc.M111.306530

FIGURE 2.

FIGURE 2.

Endotoxin priming elicits endocytosis, and endocytosis is required for priming of the respiratory burst. A, flow cytometry analysis of endocytosis of TR-dextran in control versus LOS:sCD14-treated PMN demonstrates a significant increase in endocytosis in response to priming. Fold increase presented with respect to control cells at time 0, * = p < 0.05, n = 5. B, effect of the dynamin-II inhibitor Dynasore on endocytosis of TR dextran in control versus LOS:sCD14 PMNs. Dynasore (Dyn, 300 μm) significantly inhibits constitutive endocytosis in unstimulated PMNs. Dynasore also blocked LOS:sCD14-mediated enhancement of TR-dextran endocytosis, * = p < 0.05, n = 6. C, PMN were primed with LOS:sCD14 in the absence or presence of Dynasore for 30 min before washing twice and then stimulating with fMLF. Primed NADPH oxidase activity, as measured by LUC-CL, in response to fMLF was markedly inhibited in the cells primed in the presence of Dynasore, representative tracing, n = 8. D, both chlorpromazine (40 μm) and sucrose (225 mm) significantly inhibited LOS:sCD14-primed NADPH oxidase activity, as measured by LUC-CL, in response to fMLF. ** = p < 0.05 sucrose compared to LOS:sCD14 without inhibitor, * = p < 0.05 for chlorpromazine compared to no inhibitor, n = 8.