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. 2012 Jan 10;287(15):12395–12404. doi: 10.1074/jbc.M111.306530

FIGURE 3.

FIGURE 3.

Confocal microscopy analysis of endotoxin primed PMN displays ROS-containing vesicles that colocalize with NADPH oxidase components and early endosomal markers. PMNs were incubated for 30 min with LOS:sCD14 (10 ng/ml) in the presence of the ROS-sensitive probe, Oxyburst, and Alexa Fluor 647 dextran. Oxyburst positive, ROS-containing vesicles are seen in green (A, E, I) and colocalize with gp91phox in blue (B, J), Alexa Fluor 647 dextran in blue (F), p40phox (C), p67phox (G), and rab5 (K), all in red. D, H, L, representative merged images.