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. 2012 Jan 10;287(15):12395–12404. doi: 10.1074/jbc.M111.306530

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Cell-free NAPDH oxidase assay for measurement of superoxide production using the reduction of ferricytochrome c displays a shift in the superoxide generating fractions following endotoxin priming. A, in control PMN, pooled subfractions 3 and 4 generated the greatest amount of superoxide following addition of NADPH, whereas peak superoxide generating activity shifted to subfractions 4 and 5 following endotoxin priming (B). C, compiled data for superoxide generation by subfractions from n = 3 FFE experiments comparing control versus endotoxin-primed PMN displays a full fraction shift in peak superoxide generating activity.