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. 2012 Feb 7;287(15):12417–12424. doi: 10.1074/jbc.M111.333856

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Knock-out of CIBZ in ESCs delays G1/S transition. A and B, deletion of CIBZ delays G1/S transition. The cell cycle distribution of CIBZ^−/−1 ESCs were analyzed by flow cytometry with PI staining. A, representative flow cytometry profiles show percentages in each stage of the cell cycle on day 3 after seeding cells. B, graphic representation of the percentage of cells in each phase. Results are mean ± S.E. of five independent experiments. *, p < 0.05. C, Western blotting analysis showed the expression of the indicated proteins in CIBZ^−/−1 ESCs. α-Tubulin served as loading controls.