FIGURE 5.
Constitutive ectopic expression of CIBZ promotes proliferation and G1/S transition. A, Western blotting (left panel) and semiquantitative RT-PCR (right panel) analysis of stable Myc tag and Myc-CIBZ transfected ESCs. Endo and exo indicate endogenously generated and exogenously added CIBZ, respectively. B, CIBZ stably expressing ESC line (2 × 105 cells) were seeded in a 6-well plate. The number of cells was counted before and after seeding at 2 days. Results are mean ± S.E. of three independent experiments (n = 6 in total, *, p < 0.05). C, graphic representation of the percentage of cells in each phase. Results are mean ± S.E. of five independent experiments. *, p < 0.05. D, Western blotting analysis showed the expression of the indicated proteins in Myc tag and Myc-CIBZ stable ESC line on Western blots.