FIGURE 6.

Constitutive overexpression of Nanog in ESCs rescues the compromised proliferation by siRNA-mediated CIBZ knockdown. A, Western blotting analysis of CIBZ knockdown in vector ESC line and Nanog-overexpressing ESC lines (#1 and #2). CIBZ siRNA or scrambled negative siRNA (NC) was transiently transfected into the vector and Nanog ESC lines. At 2 days after transfection, cells were collected and analyzed by immunoblotting with the indicated antibodies. B and C, analysis of proliferation of ESCs (4 × 10⁵) transfected with CIBZ siRNA or scrambled siRNA and plated in a 6-well plate. B, cell number was counted after seeding at 2 days. Results are mean ± S.E. of three independent experiments (n = 6 in total, *, p < 0.05). C, graphic representation of cell number reduction rate, which was calculated by decreased number of scrambled siRNA cells relative to CIBZ siRNA cells/number of scrambled siRNA cells. *, p < 0.05.