FIGURE 5.

The N terminus of Vpx mediates DCAF1-dependent down-regulation of SAMHD1 levels. HEK 293T cells were transiently co-transfected with plasmids expressing DCAF1, wild type, or mutant Vpx (N12A, E15A, E16A, and T17A), and/or human SAMHD1, as indicated. Each transfection was performed in duplicate. Extracts prepared from the transfected cells were separated on 10–20% SDS-PAGE and Vpx, SAMHD1, and DCAF1 were revealed by immunoblotting. A nonspecific protein band (*) cross-reacting with the DCAF1 antibody (*) is shown as a loading control.