FIGURE 1.

Body and tissue weights of Acc2^−/− mutant and control mice under different feeding conditions. The weights of 4–5-month-old male mice (n = 5) in each different feeding group, as indicated at the right of each panel, were measured. ND, mice that were maintained on a normal diet for 4–5 mo; FRFD, mice that fasted for 48 h and then fed a fat-free, high-carbohydrate diet for an additional 48 h; HFHC, 3–4-month-old male mice that were fed a high-fat, high-carbohydrate diet for an additional 2 months; FAST, 4–5-month-old mice that fasted for 48 h. Before the mice were sacrificed, blood was collected from their tails after a 4–5-h fasting period to determine the blood constituents. At the end of each experiment, the mice were weighed and sacrificed, and the extracted tissues were weighed and kept at −80 °C for further analyses.