FIGURE 2.

Representative Western blots of lipogenic enzymes, and TG and cholesterol levels in liver extracts from control and Acc2^−/− mutant mice fed a normal diet. In Western blot analyses, crude liver extracts (“Experimental Procedures”) were separated by 4–12% NuPAGE MES gels. A, 30-μg protein samples of liver extracts from control and Acc2^−/− mutant mice were separated by gel electrophoresis. Antibodies against ACL, ACC1, and FAS were used to detect their respective levels with ECL. The bottom panel shows a representative Western blot after staining with Ponceau S to indicate equal loading. B, the respective bands in the blots in A were scanned and quantified. C and D, activities of ACC and FAS in liver crude extracts, respectively. E and F, triglyceride and cholesterol levels in liver extracts from the two groups of mice were determined as described under “Experimental Procedures.”