Levels of transcription factors PPAR-γ and PPAR-α in liver extracts of control and Acc2−/− mutant mice under high-fat, high-carbohydrate feeding conditions.
A, representative Western blots of PPAR-γ and PPAR-α, and Ponceau S-stained filters as a control for equal loading. B and C, relative levels of PPAR-γ and PPAR-α as determined by scanning the respective bands. D, relative mRNA levels of PPAR-γ and PPAR-α transcripts determined by real-time quantitative PCR. Data are expressed as mean ± S.D. (n = 5; *, p < 0.05).