TABLE 1.

Serum metabolite data from wild-type and Acc2^−/− mutant mice under different dietary conditions

Blood was withdrawn from tails after a 5–6-h fasting period, and serum was separated and kept at 20 °C for further analysis of the blood constituents.

Blood constituents	NDa		FFHC		HFHC		Fast
	WT	Mut	WT	Mut	WT	Mut	WT	Mut
Glu (mg/dl)	205 ± 23	182 ± 9	151 ± 10	151 ± 9	233 ± 19	186 ± 13b	74 ± 13	71 ± 11
Cholesterol (mg/dl)	145 ± 22	154 ± 22	92 ± 15	123 ± 9b	204 ± 38	270 ± 26	177 ± 11	149 ± 10b
HDL cholesterol (mg/dl)	131 ± 33	140 ± 22	83 ± 10	104 ± 6b	186 ± 31	242 ± 35b	165 ± 11	145 ± 10
LDL cholesterol (mg/dl)	14 ± 2	18 ± 3	12 ± 11	21 ± 4b	17 ± 4	29 ± 7b	12 ± 2	11 ± 3
TG (mg/dl)	157 ± 52	101 ± 15b	91 ± 20	61 ± 4b	137 ± 41	93 ± 15b	160 ± 40	80 ± 6b
VLDL cholesterol (mg/dl)	32 ± 11	20 ± 9b	18 ± 4	12 ± 1b	28 ± 8	18 ± 3b	38 ± 12	16 ± 1b
NEFA (mEq/dl)	3.1 ± 0.3	2.3 ± 0.2b	1.1 ± 0.1	0.8 ± 0.1	2.1 ± 0.4	1.5 ± 0.2b	1.7 ± 0.1	1.2 ± 0.1b

^a ND, normal diet; FFHC, fasted-refed; Fast, fasted for 48 h; HFHC, high-fat, high-carbohydrate diet; mut, mutant; NEFA: nonesterified fatty acids; VLDL, very low-density lipoprotein.

^b Values are mean ± S.D. (n = 5), p < 0.05.