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. 2012 Apr 6;7(4):e34904. doi: 10.1371/journal.pone.0034904

Figure 2. DPI, NAC and resveratrol decrease lipolysis.

Figure 2

Human differentiated adipocytes were treated with or without (A) 10 µM DPI, (B) 5 mM NAC, or 100 µM resveratrol in the absence or presence of 5 µM forskolin for 4 hours in KRB containing 1% BSA and 0.5 mM oleate. Media were collected and assayed for glycerol using an enzyme-linked luciferase assay. Results for glycerol are normalized to the basal condition and represented as fold increase. Average basal value was 16.7 ± 1.7 nmols per million cells per hour, average forskolin-stimulated value was 85.8 ± 9.1 nmols per million cells per hour. Values ± S.E.M. for three independent experiments; * p <0.05 vs. basal control, # p<0.05 vs. forskolin control.