Figure 4. HSL phosphorylation was decreased by DPI treatment under lipolytic stimulation after 15 min, 1 hour and 4 hours.

Human differentiated adipocytes were treated with or without 10 µM DPI in the absence or presence of 5 µM forskolin for (A) 4 hours (B) 1 hour or (C) 15 min in KRB with 1% BSA and 0.5 mM oleate. Individual proteins were analyzed by western blotting with specific antibodies. Signals were visualized with an enhanced chemiluminescence substrate kit (Thermo Scientific, Rockford IL) as described in materials and methods. Immunoblots shown are representative of those obtained from three separate experiments.