Figure 7. NAC abrogated forskolin-induced HSL translocation from the cytosol to the lipid droplet.

Human differentiated adipocytes were treated as described in figure 6 except with or without 5 mM NAC for 15 minutes. Cell were then fixed with paraformaldehyde and subsequently incubated with anti-total HSL antibody (green); cells were also labeled with Lipotox red for lipid droplet detection (red). Confocal pictures were taken (A) and analyzed using image J software. (B) The panel shows normalized mean values ± S.E.M. for three independent experiments with at least 50 cells per condition per experiment; * p <0.05 vs. basal, # p<0.05 vs. forskolin.