Figure 2.

Cellular levels of CIA5 under low and high CO₂ conditions. Western blot analysis was performed with total cell lysates of three separate cia5 mutants (lanes 4 and 5; lanes 6 and 7; lanes 8 and 9, respectively) complemented with an intact, HA-tagged Cia5 gene and maintained under low CO₂ conditions (lanes 4, 6, and 8) or high CO₂ conditions (lanes 5, 7, and 9). Epitope-tagged CIA5 was detected with antibodies to HA. Specificity of the antibody for epitope-tagged CIA5 was demonstrated by the lack of detection of proteins of the expected size in extracts from cia5 mutants (lane 1) or from wild-type cells maintained in high (lane 2) or low (lane 3) CO₂ conditions.