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. Author manuscript; available in PMC: 2013 Apr 6.
Published in final edited form as: J Proteome Res. 2012 Feb 29;11(4):2609–2618. doi: 10.1021/pr201108q

Fig 3. Mild alkaline borohydride treatment of glycoproteins.

Fig 3

A: Mild alkaline borohydride treatment of [9-3H] labeled sialyl mucin glycoproteins was performed in Teflon lined screw-capped test tubes using 1.0M Na borohydride in 0.1 N NaOH in a total volume of 1.0 ml. Samples were incubated at 45°C for 24h, excess borohydride was destroyed by adding drops of acetic acid carefully, and product was fractionated on Biogel P6 column as described in Fig 2 A. Results are presented for T47D (subpanel a), MCF-7 (subpanel b) and LS180 (subpanel c). B: Thin layer chromatography (TLC) analysis of Biogel P6 fractions obtained in panel A [T47D fraction B; MCF-7 fraction B; LS180 fractions B, C] and also the major O-glycan of fetuin. TLC was carried out on Silica gel GHLF (250μm scored 20X20cm; Analtech Newark DE) with 1-propanol/NH4OH/H2O (12/2/5 v/v) as solvent. The [9-3H] sialyl products were located by scraping 0.5cm width segments of silica gel and soaking in 2.0ml water in vials followed by liquid scintillation counting.