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. Author manuscript; available in PMC: 2013 Apr 6.
Published in final edited form as: J Proteome Res. 2012 Feb 29;11(4):2609–2618. doi: 10.1021/pr201108q

TABLE 1.

Characteristics of [9-3H]sialylated glycoproteins present in human cancer cell lysates

Incorporation of [9-3H]NeuAc/mg wt* [9-3H]sialyl Glycoproteins binding Biogel P6 [9-3 H]Sialyl fractions from pronase digestion
Cancer cell line CPM x 10−4 WGA- Agarose % VVL- Agarose % P6 excluded P6 included
T47D (breast) 20.54 100 70 Minor
WB 95%
VB 5%		 Major
WB 40%
VB 40%
MCF (breast) 4.75 >90 40 Major Major
LS180 (colon) 8.31 >90 40 Major
WB 95%
VB 20%		 negligible
SKOV3 (ovary) 8.48 60 <10 Major Major
Hep G2 (hepatic) 3.35 60 <10 Major Major
HL60 (leukemia) 3.10 60 <10 Major Major
LNCap (prostate) 8.89 50 <10 Major Major
*

Extensively dialyzed and then lyophilized material from [9-3H]sialylated cancer cell lysates

WB: WGA- agarose binding and VB: VVL-agarose binding