TABLE 1.
Incorporation of [9-3H]NeuAc/mg wt* | [9-3H]sialyl Glycoproteins binding | Biogel P6 [9-3 H]Sialyl fractions from pronase digestion | |||
---|---|---|---|---|---|
Cancer cell line | CPM x 10−4 | WGA- Agarose % | VVL- Agarose % | P6 excluded | P6 included |
T47D (breast) | 20.54 | 100 | 70 | Minor WB 95% VB 5% |
Major WB 40% VB 40% |
MCF (breast) | 4.75 | >90 | 40 | Major | Major |
LS180 (colon) | 8.31 | >90 | 40 | Major WB 95% VB 20% |
negligible |
SKOV3 (ovary) | 8.48 | 60 | <10 | Major | Major |
Hep G2 (hepatic) | 3.35 | 60 | <10 | Major | Major |
HL60 (leukemia) | 3.10 | 60 | <10 | Major | Major |
LNCap (prostate) | 8.89 | 50 | <10 | Major | Major |
Extensively dialyzed and then lyophilized material from [9-3H]sialylated cancer cell lysates
WB: WGA- agarose binding and VB: VVL-agarose binding