Figure 4.

Knockdown of MPPβ leads to full-length PINK1 accumulation at the outer mitochondrial membrane. (A) Mitochondria from CCCP-treated or siRNA-transfected HEK293T cells were incubated with increasing concentrations of proteinase K (PK). (B) Mitochondria from CCCP-treated or MPPβ knockdown cells were incubated ±0.1 M Na₂CO₃ before supernatant (S) and pellet (P) were separated by centrifugation. CCCP, carbonyl cyanide m-chlorophenyl hydrazone; MPP, mitochondrial processing peptidase; NT, non-targeting; PARL, presenilin-associated rhomboid-like protease; PINK1, phosphatase and tensin homologue-induced kinase 1; siRNA, short interfering RNA; WB, western blot.