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. 2012 Jan 20;31(6):1494–1505. doi: 10.1038/emboj.2011.500

Figure 1.

Figure 1

Inactivation of Mtg16 decreases HSC numbers. Quantification of flow cytometry of whole bone marrow cells. Lineage-negative cells were gated (A) and further analysed using anti-Sca-1 and anti-c-Kit (B; LSK). The LSK cells were further fractionated using anti-Flt3 to obtain the LSK/Flt3− (C), which was further divided using anti-CD150/anti-CD48 to identify the LSK/Flt3−/CD150+/CD48− populations (D). Quantification of each of these populations is calculated for the number of cells per leg (femur+tibia) and is shown to the right of each dot plot (wild type n=5, empty bars; Mtg16-null n=5, full bars). Data are expressed as mean±the standard error of the mean (s.e.m.). An unpaired two-tailed t-test indicated that the changes observed in the number of cells were significant (***P⩽0.0001).