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. 2012 Feb 7;31(7):1714–1726. doi: 10.1038/emboj.2012.19

Figure 5.

Figure 5

The MDA5 helicase and CTD domains do not associate with the CARDs and bind RNA as a dimer. (A) The MDA5 CARDs and CARD-deleted MDA5 elute separately from a size-exclusion column (blue). Elution curves of the two components loaded separately are shown in grey. (B) Plateau in the Kratky plot of full-length MDA5 indicates substantial flexibility within the molecule. (C) Histograms of the radius of gyration (Rg) and maximum dimension (Dmax) of full-length MDA5 models (orange) selected to fit the SAXS data with a five-curve minimal ensemble from a pool of 105 random domain arrangements (black). (D) Single-phase DA model of CARD-deleted MDA5 in complex with AU20 using data to 0.168 Å−1 (average of 15 models). (E) Three-phase DA model (average of seven) of the same complex as in (D) with two protein phases (cyan and green) and one RNA phase (magenta). (F) Rigid-body model using knowledge-based restraints of the same complex as in (D, E). See Supplementary Figures S4 and S5.