Gene organization of Ccm1. The solid rectangles indicate
the position of the protein-coding regions. The insertion site of the
Nia1 gene in the C16 mutant is depicted by a large open
triangle with an arrow. Positions of the oligonucleotide probes (C16–5
and C16–3) are shown by small triangles. Positions of a probe for the
1.5-kb EcoT22I DNA fragment (P-1) corresponding to the
5′-upstream region of Nia1, and
Ccm1-specific probes (P-3 and P-5) are indicated by
thick bars. A 4.8-kb BamHI fragment is between two
BamHI sites. A, ApaI; B,
BamHI; S, SacI; X, XhoI;
(A)n, poly(A) tail.