Figure 2.

Complementation of C16 by transformation. (A) Relative positions of a genomic clone, pKI4, and its derivatives. Transformants of C16 cells, which photoautotrophically grew in ordinary air, are indicated by a +. (B) Northern blot analyses of Cah1 expression. Ten micrograms of total RNA from cells grown under high-CO₂ (HC) or low-CO₂ (LC) conditions were hybridized by a ³²P-labeled Cah1-specific oligonucleotide probe. (C) Southern blot analysis of BamHI-digested total genomic DNA from WT, C16, and C16∷pKI4 cells. The blot was hybridized with a ³²P-labeled Ccm1-specific probe, P-5.