Figure 6.

Expression of Ccm1 gene. (A) Ten micrograms of poly(A)⁺ RNA from WT in high-CO₂ (lanes 1), WT in low-CO₂ (lanes 2) and C16 in low-CO₂ (lanes 3) were electrophoresed and hybridized with ³²P-labeled Ccm1-specific probes P-5 (A a) and P-3 (A c). The same blot was reprobed by a ³²P-labeled DNA fragment designated P-1 in Fig. 1 (A b) and by a Cblp cDNA (A d). (B) High-CO₂-grown WT cells (lane 1), low-CO₂-grown WT cells (lane 2), and low-CO₂-grown C16 cells (lane 3) were labeled with [³⁵S]-methionine and [³⁵S]-cysteine, and immunoprecipitated with anti-CCM1 antibody. As a control, low-CO₂-grown WT cells were labeled and immunoprecipitated with rat normal serum (lane 4).